In vivo gene regulation using tetracycline-regulatable systems
نویسندگان
چکیده
منابع مشابه
Homogeneous tetracycline-regulatable gene expression in mammalian fibroblasts.
The expression of transfected genes in mammalian cells is rapidly repressed by epigenetic mechanisms such that, within a matter of weeks, only a fraction of the cells in most clonal populations still exhibit detectable expression. This problem can become prohibitive when one wants to express two ectopically introduced genes, as is necessary to establish cell lines that harbor genes regulated by...
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Conventional tools for elucidating gene function are relatively scarce in Candida albicans, the most prevalent human fungal pathogen. To this end, we developed a convenient system to control gene expression in C. albicans by the tetracycline-regulatable (TR) promoters. When the sea pansy Renilla reniformis luciferase gene (RLUC1) was placed under the control of this system, doxycycline (DOX) in...
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ژورنال
عنوان ژورنال: Advanced Drug Delivery Reviews
سال: 2009
ISSN: 0169-409X
DOI: 10.1016/j.addr.2008.12.016